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In mycology, anastomosis is the fusion between branches of the same or different hyphae. Hence the bifurcating fungal hyphae can form true reticulating networks. By sharing materials in the form of dissolved ions, hormones, and nucleotides, the fungus maintains bidirectional communication with itself. The fungal network might begin from several origins; several spores, several points of penetration, each a spreading circumference of absorption and assimilation. Once encountering the tip of another expanding, exploring self, the tips press against each other in pheromonal recognition, fusing to form a genetic singular that can cover hectares called a genet.

For fungi, anastomosis is also sex. In some fungi, two different haploid mating types - if compatible - merge. Somatically, they form a morphologically similar mycelial wave front that continues to grow and explore. The significant difference, is that in each septated unit is binucleate, containing two unfused nuclei, i.e. one from each parent that do not undergo karyogamy.